APPLE-MS: A affinity purification-mass spectrometry method assisted by PafA-mediated proximity labeling

While affinity purification-mass spectrometry (AP-MS) has significantly advanced protein-protein interaction (PPI) studies, its limitations in detecting weak, transient, and membrane-associated interactions remain. To address these challenges, we introduced an innovative proteomic method termed Affinity Purification coupled Proximity LabEling-Mass Spectrometry (APPLE-MS), which combines the high specificity of Twin-Strep-tag enrichment with PafA-mediated proximity labeling. This method achieves … Read more

Ozempic Has Caused Thousands to Lose Weight Dramatically—Now Their Closets Are for Sale

Novo Nordisk, the maker of Ozempic, just released its first-quarter results, and they’re eye-popping: $4.4 billion in revenue, up 14% from the same period last year. That surge has sent the company’s stock price soaring. But the more interesting story is why. The answer is simple: demand for obesity treatments is up 67%. The Ozempic … Read more

The truth about GLP-1 medications for weight loss: What every patient should know

For years, weight loss has been sold as a simple math problem: eat less, move more, and the pounds will melt away. But if that were true, we wouldn’t have an obesity epidemic affecting over 40 percent of adults in the U.S. Now, a new class of medications—GLP-1 receptor agonists like semaglutide (Ozempic, Wegovy) and … Read more